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. 2022 Sep 5;5(11):e202201570. doi: 10.26508/lsa.202201570

Figure S1. Sequential phosphorylation of CiC by Fu and CK1.

Figure S1.

(A, B) In vitro kinase assay was carried out in which GST-CiCWT and GST-CiCS1385A (A) or GST-CiC2SA (B) were incubated with CC-FuEE purified from insect cells. After removal of the Fu kinase, the GST fusion proteins were incubated with or without a recombinant CK1, followed by Western blot analysis using the indicated antibodies.