Table 2.
Information of sequences of the oligonucleotide primers.
| Genes1 | Genbank number | Primer sequences (5′→to 3′ direction) | Product size (bp) |
|---|---|---|---|
| GAPDH | NM_204305 | Forward: GAGGGTAGTGAAGGCTGCTG | 113 |
| Reverse: CATCAAAGGTGGAGGAATGG | |||
| GRP78 | NM_205491.1 | Forward: TCCTGCTCCTCGTGGTGTCC | 148 |
| Reverse: CTCCTCTGGTGTTAGCCGATTCTG | |||
| PERK | XM_420868.6 | Forward:GTGGATGAGCAGGAGGCAATGATG | 150 |
| Reverse:ATCCTTAACCAGCCATGCAGAAGC | |||
| EIF2A | NM_001031323.2 | Forward: GCTGCGAGTCAGTAATGGGTATAA | 103 |
| Reverse: CTGCCAGGAAACTTGCCACA | |||
| ATF4 | NM_204880.2 | Forward: AATTGGCTCGCTGTGGACAG | 148 |
| Reverse: CGGTGGCTTCCAGATGTTCC | |||
| ATF6 | XM_422208 | Forward: GATTGTGGGCGTCACTTCTCG | 142 |
| Reverse: TGGGATGCCAATGTTAGCCTG | |||
| IRE1 | NM_001285499 | Forward: TGAGGGCAATGAGAAATAAGAAGC | 127 |
| Reverse: TGTAGGAGCAGGTGAGGGAAGC | |||
| XBP1 | NM_001006192 | Forward:GCGAGTCTACGGATGTGAAGGA | 140 |
| Reverse: TGTGGAGGTTGTCAGGAATGGT | |||
| CHOP | XM_015273173.2 | Forward:CAGGAAGAAGAGCTGGCCCCACT | 123 |
| Reverse:TGCTGTGCTCGCCGTGCTGT | |||
| Caspase-3 | XM_015276122.2 | Forward:ACAGCAAGCGAAGCAGTTTT | 115 |
| Reverse:TCACCTCTGAAAAGGCTGGT | |||
| Bcl-2 | NM_205339.2 | Forward:ATCGTCGCCTTCTTCGAGTT | 150 |
| Reverse:ATCCCATCCTCCGTTGTCCT |
1
Abbreviations: ATF4, Activated transcription factor 4; ATF6, Activated transcription factor 6; Bcl-2, B-cell CLL/lymphoma 2; CHOP, transcriptional factor C/EBP homologous protein; EIF2A, elongation initiation factor 2 alpha; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GRP78, glucose-regulated protein 78; IRE1, Inositol-requiring enzyme 1; PERK, protein kinase RNA-like ER kinase; XBP1, X-boxbinding protein 1.