Association between immune cell infiltration, tumor mutation burden, and E2F7 expression. (A) Association between E2F7 expression and immune infiltration levels in KIRC. (B-D) Association between the expression of RP11–478C19.2, hsa-miR-181b-5p, hsa-miR-181a-5p, hsa-miR-181c-5p, and E2F7 and immune checkpoint gene expression. (E) Relative expression of PD-L1 between the si-E2F7 and si-NC groups in OSRC-2 and 786-O cells. (F) Comparison of tumor mutation burden between the E2F7high and E2F7low groups. (G) Preliminary validation in GSE67501 (* p < 0.05, ** p < 0.01, *** p < 0.001).