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. 2022 Aug 23;12:967796. doi: 10.3389/fcimb.2022.967796

Figure 2.

Figure 2

The activity of the purified enzyme r-BcsZ. (A) r-BcsZ enzyme was expressed in E. coli, purified, and spotted on CMC agar plates. The plates were stained with 0.1% Congo red and washed with 1M NaCl. A bright halo is observed, indicating CMC degradation. (B) Glucanase specific activity of r-BcsZ measured with BCA assay using CMC and Avicel as substrates.