Figure 5.

Necrotic damage on parsley leaves infiltrated with different Salmonella strains and solutions. (A) Summary of the necrotic damage intensity 7 days post infiltration. The damage in each leaf was scored according to a damage gradient described in Figure S2 , and the mean scores of all leaves were calculated. The experiment was conducted in 3 repeats and statistical calculations were performed using Kruskal-Wallis test. **P < 0.001. Error bars represent standard deviations. B-H. Images of representative leaves. (B) Parsley leaves 7 days after bacterial infiltration of w.t., no leaf damage was observed. (C) Parsley leaves 7 days after pBcsZ infiltration, necrotic damage on leaves was observed. (D) Parsley leaves 7 days after bacterial infiltration of ΔBcsZ, no damage was observed. (E) Parsley leaves infiltrated with the purified r-BcsZ, necrotic leaf damage was observed. (F) Parsley leaves Infiltrated with buffer solution as control. (G) Parsley leaves infiltrated with degradation products of BcsZ, necrotic leaf damage was observed. (H) Infiltration of the denaturized enzyme r-BcsZ, no damage was observed. Red arrows point to the necrotic spots formed in the parsley leaf.