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. 2022 May 28;24(5):222–229. doi: 10.22074/cellj.2022.7763.

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Fig.2 — Knockdown of DSCAM-AS1 inhibited OSCC cell proliferation, migration, and invasion. A. After transfection of DSCAM-AS1 siRNA, DSCAM-AS1 was downregulated in HSC-3 and CAL-27 cells, which was verified by qRT-PCR. B, C. MTT assay showed that OSCC cell proliferation was lower in the si-DSCAM-AS1#1 group. D. EdU assay showed that detect OSCC cell proliferation level was lower in the si-DSCAM-AS1#1 group. E, F. Transwell assay showed that OSCC cell migration and invasion abilities were decreased in the si-DSCAM-AS1#1 group. The experiments were performed in triplicate. *; P<0.05, **; P<0.01, ***; P<0.001, OSCC; Oral squamous cell carcinoma, qRT-PCR; Quantitative real time polymerase chain reaction, siRNA; Small interfering RNA, MTT; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and EdU; 5-ethynyl-2’-deoxyuridine.