FIG. 5.
Concentration dependence of the fluorescence increase at 315 nm induced by peptides binding to OppA(WT) (●), OppA(D471R) (○), and OppA(A477D) (▾) in the presence of bradykinin (RPPGFSPFR) (A) and SLSQSKVLP (B). The concentration of all OppA proteins in this experiment was 0.6 μM. The solid line through the data points represents the best fit. The Kd values obtained for OppA(WT) were 0.29 ± 0.02 for bradykinin and 2.07 ± 0.07 for SLSQSKVLP. The Kd values for OppA(D471R) were 2.83 ± 0.09 for bradykinin and 2.26 ± 0.05 for SLSQSKVLP. The Kd values for OppA(A477D) were 20.86 ± 0.56 for bradykinin and 6.22 ± 0.13 for SLSQSKVLP.