TABLE 2.

Oligonucleotides used for cloning and mutagenesis^a

Primer	Oligonucleotide	Characteristic
FBB	5′-CGCGGATCCGAGCCCTCCTGCCGGAT-3′	BamHI
RCP	5′-AAAACTGCAGGTTCATCTTAGTTTCTCC-3′	PstI
FOP	5′-CAGAAATTCCTGCAGACAAACC-3′	PstI
ROS	5′-TCACATGCATGCGGGAAACCAATGAAAGG-3′	SphI
FSA	5′-CAAGCCATGGCCAAATTAAAAGTAACTTTA-3′	NcoI
FA	5′-CAAGCCATGGGGTTCTAATCAAAGCTCA-3′	NcoI
RAB	5′-CGGTGGATCCTTTGGTGGCCAACT-3′	BamHI
FP32S	5′-GGAAGGCCTAATTCGGTCCTCGGG-3′	StuI
RP32	5′-CGATTGCCATGGCAAAATTCCTCCGA-3′	NcoI
XbaF	5′-GTTGCTCTAGATAAAGAGTC-3′	XbaI
K349QF	5′-GTGGATAATCAATTCTCAAACG-3′	K349Q
K349QR	5′-CCGTTTGAGAATTGTTATCCAC-3′	K349Q
N422HF	5′-GGTGATGCACATGCTGAAACC-3′	N422H
N422HR	5′-GGTTTCAGCATGTGCATCACC-3′	N422H
A423HF	5′-GATGCAAACCATGAAACCATTGC-3′	A423H
A423HR	5′-GCAATGGTTTCATGGTTTGCATC-3′	A423H
D471RF	5′-GGATATCACTCGTGGTTCTTGG-3′	D471R
D471RR	5′-CCAAGAACCACGAGTGATATCC-3′	D471R
A477DF	5′-GGTCATTGGATTCTGAACC-3′	A477D
A477DR	5′-GGTTCAGAATCAATGACC-3′	A477D

^aNucleotides in boldface type correspond to restriction site; nucleotide changes that give rise to an amino acid substitution are underlined.