TABLE 2.
Oligonucleotide PCR primers
| Primer set | Forward primera | Reverse primerb | Description |
|---|---|---|---|
| A | NNNCATATGTTAAATCAGAGATTAGAAGTAAGG | NNNGGATCCTTAAATCCTTCTTTCCCTTGAGGAGGG | A. aeolicus rpoN coding sequence |
| B | NNNCATATGGATTTAAAGGTAGAGATCGAAACT | GTGCGACAGAACGGATCCTTACTC | A. aeolicus nifA coding sequence |
| C | CCACATCATCACACAATCG | CAGACTTCATAGCATTTCC | E. coli glnHp2 and glnHp2-m11 promoters |
| D | GGCGTAAACTATTTTGTAATC | ACCCATACTTCTTACCTCCATT | A. aeolicus dhsU upstream region |
| E | CTTCCTCGCCGTGTTAGAA | TCATCTTCCTTCCTCCTTGTAAGTT | A. aeolicus glnB upstream region |
| F | CCCGTGGGAACACCTTCGAGT | CATTCTTTCACTCCTCCTGAG | A. aeolicus nirB upstream region |
| G | AAACACGAAAAATTTCCATACCTT | GAGGGCGTCCTTCACTTCGTCG | A. aeolicus glnB region 147-bp fragment |
| H | GGAAATTAAGCTGATTTAGTACC | GAGGGCGTCCTTCACTTCGTCG | A. aeolicus glnB region 203-bp fragment |
a
NdeI site is underlined.
b
BamHI site is underlined.