TABLE 3.
Specific activities of isocitrate lyase, IDH, and citrate synthase from csrA+ and csrA::kanR strains grown under different conditionsa
| Medium | Sp act (U/mg of protein ± SD; n ≥ 3)
|
|||||
|---|---|---|---|---|---|---|
| Isocitrate lyase
|
IDH
|
Citrate synthase
|
||||
| csrA+ | csrA::kanR | csrA+ | csrA::kanR | csrA+ | csrA::kanR | |
| MOPS | ||||||
| Acetate and succinate | 0.083 ± 0.006 | 0.041 ± 0.006 | 1.23 ± 0.22 | 1.27 ± 0.05 | 0.61 ± 0.05 | 0.51 ± 0.05 |
| Glucose | 0.008 ± 0.000 | 0.010 ± 0.000 | 1.27 ± 0.12 | 1.24 ± 0.08 | 0.20 ± 0.01 | 0.16 ± 0.00 |
| Kornberg + glucose | 0.003 ± 0.000 | 0.003 ± 0.000 | 0.47 ± 0.03 | 0.49 ± 0.04 | 0.03 ± 0.00 | 0.03 ± 0.00 |
a
Cultures were grown in supplemented MOPS medium containing the indicated carbon sources or in Kornberg medium to mid-exponential phase. Enzyme assays were conducted as described in Materials and Methods.