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. 2022 Aug 23;12:992214. doi: 10.3389/fcimb.2022.992214

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Copyright © 2022 Cramer, Nawrot, Wege, Dorda, Sommer, Danov, Wronski, Braun, Jonigk, Fischer, Munder and Tümmler

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Outcome of competitive fitness experiments in mPCLS and hPCLS after 4 h and 8 h. Serial P. aeruginosa isolates from mild courses (upper panel) and severe courses (lower panel) were grouped by colonization time in CF patients’ lungs time in early, intermediate and late isolates (see Supplementary Tables S4 / S5 for details). Relative growth changes are shown for the individual strains of the three groups. The number of data points per group depends on the number of isolates assigned to that group. Since early/late isolates were assigned to the first/last quartile of the infection period, respectively, the intermediate isolates extend to the inner two quartiles and are therefore quantitatively superior. Statistics and thus the significance were calculated with the help of Kruskal-Wallis. All p-values were corrected for multiple testing by Bonferroni correction.