TABLE 1.
Plasmids used in this study
| Plasmid | Characteristics or construction | Source or reference |
|---|---|---|
| pCR 2.1 | E. coli PCR cloning vector; bla kan | Invitrogen |
| pET9d | E. coli protein expression vector; PT7kan | Novagen |
| pRDC19 | B. subtilis thrC integration vector; Pxyl xylR erm spc bla | F. Arigoni |
| pNG7 | B. subtilis soj/spoOJ integration vector; bla spc | 13 |
| pLP75 | pCR2.1 plus 629-bp PCR product using prfA-NcoI and prfA-BamHI | This work |
| pLP76 | pET9d plus 629-bp NcoI-BamHI fragment from pLP75 | This work |
| pLP78 | pCR2.1 + 670-bp PCR product using prfA-SphI and prfA-B/S | This work |
| pLP79 | pRDC19 + 668-bp SphI-BamHI fragment from pLP78 | This work |