pCR 2.1 |
E. coli PCR cloning vector; bla kan
|
Invitrogen |
pET9d |
E. coli protein expression vector; PT7kan
|
Novagen |
pRDC19 |
B. subtilis thrC integration vector; Pxyl xylR erm spc bla
|
F. Arigoni |
pNG7 |
B. subtilis soj/spoOJ integration vector; bla spc
|
13 |
pLP75 |
pCR2.1 plus 629-bp PCR product using prfA-NcoI and prfA-BamHI |
This work |
pLP76 |
pET9d plus 629-bp NcoI-BamHI fragment from pLP75 |
This work |
pLP78 |
pCR2.1 + 670-bp PCR product using prfA-SphI and prfA-B/S |
This work |
pLP79 |
pRDC19 + 668-bp SphI-BamHI fragment from pLP78 |
This work |