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. 2022 Sep 6;10(17):e15429. doi: 10.14814/phy2.15429

FIGURE 3.

FIGURE 3

Immunofluorescence of occludin and vimentin. Histologic sections (×400) of the axial and alveolar staining for immunofluorescence to occludin and vimentin divided by groups (CF‐PCV, CF‐PSV, LF‐PCV, and LF‐PSV). The arrows indicate the endothelial cells of the bronchiolar and alveolar capillaries expressing occludin [nuclei in blue, 4′,6‐diamidino‐2‐phenylindole (DAPI); connective tissue in green; vimentin; bronchiolar vessels and alveolar capillaries in red; occludin and colocation, blue‐orange‐red]. (a) DAPI (nuclei in blue) in axial parenchyma in CF‐PCV. (b,f) Vimentin in axial parenchyma in CF‐PCV. (d) merge of DAPI, vimentin and occludin in axial parenchyma in CF‐PCV. (e) DAPI (nuclei in blue) in alveolar parenchyma in CFPCV. (f) Vimentin in alveolar parenchyma in CF‐PCV. (h) Merge of DAPI, vimentin and occludin in alveolar parenchyma in CF‐PCV. (i) DAPI (nuclei in blue) in axial parenchyma in CF‐PSV. (j) Vimentin in axial parenchyma in CF‐PSV. (l) Merge of DAPI, vimentin and occludin in alveolar parenchyma in CF‐PCV. (m) DAPI (nuclei in blue) in alveolar parenchyma in CF‐PSV. (n) vimentin inalveolar parenchyma in CF‐PSV. (p) Merge of DAPI, vimentin and occludin in alveolar parenchyma in CF‐PSV. The CF‐PCV parenchyma (C and G) presents intense red birefringence of occludin in dilated lymphatics and blood vessels around the bronchiolar mucosa and along the capillary alveoli (simple white arrows). CF‐PSV and LF‐PCV (K, O, C2, and G2) present a similar pattern of birefringence for occludin (red) in endothelial cells of the lymphatics and blood vessels (double white arrows), as well as along capillaries of the alveolar septa (single white arrows). In contrast, LF‐PSV (K2 and O2) has almost no birefringence of occludin in the endothelium of the lymphatics and blood vessels, as well as along capillaries of the alveolar septa, with prominent oedema in this group (*). Br, bronchioles; Alv, alveoli; Ve, blood vessels; Cap, capillaries. White double arrows, connective tissue around bronchiolar vessels and alveolar capillaries. Simple white arrows, expression of occludin by endothelial cells of bronchiolar vessels and alveolar capillaries; white arrowhead, colocation of vimentin and occludin; *, peribronchiolar and alveolar cuff.