TABLE 1.
Bacterial strains, phage, and plasmids
| Strain, phage, or plasmid | Relevant genotype or description | Vector | Source or reference |
|---|---|---|---|
| Strain | |||
| ES1301 | lacZ53 mutS201::Tn5 thyA36 rha5 metB1 deoC | Promega | |
| GY7066 | metB thi pyrE lacMS286 (φ80dIIlacBKI) Δ(srl-recA)306::Tn10 sfiB114 | 8 | |
| JAM267 | JL2460/pJAM13 | This work | |
| JAM444 | JL2460/pJAM92 | This work | |
| JAM485 | GY7066/pJAM93 | This work | |
| JL783 | AB1157 lexA+ Δ(srl-recA)306::Tn10/F′ lacIq | 31 | |
| JL1434 | lexA71::Tn5 sulA211 supE Δ(lacIPOZYA)169 pro+/F′ lacIq lacZΔM15::Tn9 | 25 | |
| JL1447 | lexA71::Tn5 sulA211 supE Δ(lacIPOZYA)169 pro+ Δ(srl-recA)306::Tn10 (λsulA::lacZ cIind−)/F′ lacIq lacZΔM15::Tn9 | 52 | |
| JL2460 | lexA71::Tn5 sulA211 umuDC+ supE Δ(lacIPOZYA)169 pro+ Δ(srl-recA)306:: Tn10/F′ lacIq lacZΔM15::Tn9 | This worka | |
| Phage λ MMS885 | b1453 Δ(att-gam) Spi− χDcI857 | K. Knight | |
| Plasmids | |||
| pAlter | Plasmid for mutagenesis | pBR322 | Promega |
| pATT49 | lacp/o::lexAEK45 | pBR322 | A. Thliverisb |
| pBR322 | Cloning vector; bla tet | 3 | |
| pET5a UmuD | T7p::umuD | pET5a (pBR322 derivative) | G. Walker |
| pET5a UmuD′ | T7p::umuD′ | pET5a | G. Walker |
| pFG600 | lacp/o::λ cI | pBR322 | F. Gimble |
| pGB2 | Low-copy-number vector; Spcr | 4 | |
| pJAM13 | lacp/o::λ cI | pGB2 | This work |
| pJAM20 | Middle portion of recA | pAlter | This work |
| pJAM30 | C-terminal portion of recA | pAlter | This work |
| pJAM50 | N-terminal portion of recA | pAlter | This work |
| pJAM92 | lacp/o::lexAEK45 | pGB2 | This work |
| pJAM93 | lacI+ | pGB2 | This work |
| pJWL319 | lacI+ | pBR322 | This work |
| pTRecA220 | tacp/o::recA+ bla | pBR322 | 49 |
a
JL1434 was transduced to tetracycline resistance by P1 grown on JL1447.
b
pATT49 is an EK45 derivative of pJWL184 (24) made by A. Thliveris (personal communication) by replacing the MluI-BamHI interval of pJWL184 with a fragment carrying the EK45 mutation.