Table 1.
PKA pathway phosphoproteomic changes in STK25−/− compared with wild type
| Function | Protein descriptions | Fold change | Phosphorylation sites | Protein accessions | ||
|---|---|---|---|---|---|---|
| PKA | regulatory subunits | PRKAR1A | cAMP-dependent protein kinase type I alpharegulatory subunit | 0.17 | S77, S83 | P10644 |
|
| ||||||
| PRKAR2A | cAMP-dependent protein kinase type II-alpha regulatory subunit | 3.03 | S78 | P13861 | ||
|
| ||||||
| catalytic subunit | PRKACA | cAMP-dependent protein kinase catalytic subunit alpha | 0.79 | S339 | P17612 | |
|
| ||||||
| PKA targets | contractility Ca2+ handling | MYBPC3 | myosin-binding protein C, cardiac type | 100.03 | T498 | Q14896 |
|
| ||||||
| TNNT2 | troponin T, cardiac muscle | 6.18 | S132 | P45379 | ||
|
| ||||||
| 5.75 | S285 | |||||
|
| ||||||
| 5.02 | S189 | |||||
|
| ||||||
| RYR2 | isoform 2 of ryanodine receptor 2 | 2.51 | S2808 | Q92736 | ||
|
| ||||||
| 2.14 | S4368 | |||||
|
| ||||||
| CACNA1C | isoform 2 of voltage-dependent L-type calcium channel subunit alpha 1C | 1.65 | S1756 | Q13936 | ||
|
| ||||||
| energy metabolism | PHKG2 | phosphorylase b kinase gamma catalytic chain | 3.48 | T325 | P15735 | |
| GSK3α | glycogen synthase kinase-3 alpha | 2.50 | S21 | P49840 | ||
|
| ||||||
| gene expression | CREB1 | cyclic AMP-responsive element-binding protein 1 | 0.79 | S271 | P16220 | |
Fold change represents a ratio of STK25−/−/STK25+/+. Phosphorylation sites, description, and accession numbers for each uniprot ID are listed. All changes met a significance threshold of a false discovery rate (FDR)-corrected q value <0.05.