FIGURE 4. Differential MG surface phenotype after adoptive transfer of CD11b^high or CD11b^low B cells into young naïve host.

(A) Schematic. Splenocytes from naïve and stroked Ag male C57BL/6 mice underwent cell sorting to isolate CD11b^high and CD11b^low B cells. Cells were then adoptively transferred to Yg Pepboy recipient mice via retroorbital injection to contribute to modulation of MG phenotype. (B) Surface profile of MG from Yg recipient mice after adoptive transfer from Ag stroke donors. CD45 and CD11b MFI had no significant difference in MG between vehicle control and CD11b^high or CD11b^low B cell recipients. Tmem119 MFI increased in MG in CD11b^high and CD11b^low B cell recipients compared to vehicle controls (CD11b^high p=0.0014 and CD11b^low p=0.0001). P2RY12 increased in MG in CD11b^high and CD11b^low B cell recipients compared to vehicle controls (CD11b^high p=0.0040 and CD11b^low p=0.0032). MHCII MFI had no significant difference in MG between CD11b^high and CD11b^low B cell recipients and vehicle control. Experiments were performed once. Unpaired one-way ANOVA with Tukey’s multiple comparisons test, mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S. not significant.