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. Author manuscript; available in PMC: 2022 Sep 6.
Published in final edited form as: Cell Rep. 2022 Aug 16;40(7):111225. doi: 10.1016/j.celrep.2022.111225

Figure 6. IRAK4 scaffold is required for TRAF6 activation, while IRAK1 and IRAK2 show redundancy.

Figure 6.

(A) Immunoblot of ubiquitinated TRAF6 and IRAK1 pulled down from WT BMDMs treated with LPS or R848 for up to 60 min.

(B–D) Immunoblot of ubiquitinated TRAF6 and IRAK1 pulled down from indicated BMDMs treated with LPS for 30 min or R848 for 15 min.

(E) Immunoblot of ubiquitinated TRAF6 and IRAK1 pulled down from indicated BMDMs treated with LPS for up to 60 min. All stimulations were done with LPS 100 ng mL−1 or R848 1 μg mL−1. Images are representative of two (E) or three (A–D) independent experiments.