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. Author manuscript; available in PMC: 2022 Sep 6.
Published in final edited form as: Cell Rep. 2022 Aug 16;40(7):111225. doi: 10.1016/j.celrep.2022.111225

Figure 7. IRAK4 scaffold is essential to the response against Gram-negative bacteria, while IRAK-1 and -2 show redundancy.

Figure 7.

(A–F) Production of TNF-α (A, D), IL-12 (B, E), and CCL5 (C, F) in BMDMs infected with E. coli Bort for up to 24 h at MOI 1. (A–C) WT, Irak1−/−, Irak2−/−, and Irak1−/−Irak2−/− BMDMs. (D–F) WT, Irak4 Ki, Trif−/−, Irak4 Ki/Trif−/−, and Irak4−/− BMDMs.

(G–K) Immunoblot of p-ERK, p-JNK, p-p38, p-RelA, and β-actin from WT, Irak1−/−Irak2−/−, and Irak4−/− BMDMs infected with E. coli Bort at MOI 1 for up to 60 min (G) and densitometric analysis (H–K).

(L) Production of IFN-γ in splenocytes infected with E. coli Bort for 24 h at MOI 1.

(M and N) Viable intracellular bacteria counts (M) and NO production (N) in IFN-γ-primed BMDMs (100 ng mL−1, 20 h) infected with E. coli Bort for 6 h at MOI 10. (G) Image is representative of three independent experiments. Data from three (A–F, H–K, M, and N) or two (L) independent experiments (mean and SEM). *p < 0.05 in comparison with WT (one-way analysis of variance with Tukey’s multiple comparisons test).