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. 2000 Mar;182(6):1680–1692. doi: 10.1128/jb.182.6.1680-1692.2000

FIG. 5.

FIG. 5

PAN produced in M. jannaschii. Proteins were separated by reducing SDS–12% PAGE and analyzed by Western blot using anti-PAN(Δ1–73) antibodies. Lanes: 1, total protein (1 to 2 μg) produced in recombinant E. coli/pMjPAN2 which synthesizes PAN proteins of 47, 39, and 30 kDa; 2, M. jannaschii cell lysate (20 μg) which produces only the full-length PAN of 47 kDa.