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. 2022 Sep 5;13:453. doi: 10.1186/s13287-022-03150-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Expressions of hsa_circ_0001485 in BMSC osteogenic differentiation. A KEGG analysis of differentially expressed circRNA host genes in the BMSC-14d group. B GO enrichment analysis of differentially expressed circRNA host genes in the BMSC-14d group. C Analysis of TGFβ and BMP pathways using GSEA. D qPCR was used to verify the circRNA expression of BMSCs at 0, 3, 7, and 14 d of induction. E The structure of hsa_circ_0001485. F The agarose gel electrophoresis detects the form of hsa_circ_0001485. G Sanger sequencing results of hsa_circ_0001485, in which the arrow indicates the cyclization site. H qPCR detect has the expression of hsa_circ_0001485 in BMSCs and MG63 and hFOB 1.19 cells. The results are representative data from three replicates, and the data mean ± SD (ns, no statistical significance; *P < 0.05; **P < 0.01; ***P < 0.001)