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. 2022 Sep 5;13:453. doi: 10.1186/s13287-022-03150-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Hsa_circ_0001485 promoted osteogenic differentiation in the osteogenic hFOB 1.19 cells. A qPCR evaluated efficiency of hsa_circ_0001485-targeted siRNA (si-circ1485) after 48 h siRNA transfection in hFOB 1.19 cells. B Alizarin red (AR) and ALP staining of osteogenic hFOB 1.19 cells transfected with si-circ1485. For AR, magnification, 100 ×, scale bar = 200 μm; for ALP, magnification, 200 ×, scale bar = 100 μm. C ALP activity assay in osteogenic hFOB 1.19 cells transfected with si-circ1485. D, E The protein levels of RUNX2, OPN, and OCN were detected via western blot after transfection with si-circ1485 and quantification by densitometric analysis. F Osteogenic hFOB 1.19 cells overexpressing hsa_circ_0001485 (OE-circ1485) were constructed by plasmid and confirmed by qPCR assay. G AR staining and ALP staining of osteogenic hFOB 1.19 cells transfected with OE-circ1485. For AR, magnification, 100 × , scale bar = 200 μm; for ALP, magnification, 200 ×, scale bar = 100 μm. . H ALP activity assay in cells transfected with OE-circ1485. I, J After transfection with OE-circ1485 and quantification by densitometric analysis, Western blot detected RUNX2, OPN, and OCN levels. The results are representative data from three replicates, and the data mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)