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. 2022 Sep 5;13:453. doi: 10.1186/s13287-022-03150-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Hsa_circ_0001485 bound to BMPR2 to activate the BMP signaling pathway in the osteogenic hFOB 1.19 cells. A Design of the pattern diagram of the hsa_circ_0001485 probe for the pull-down test. B Silver staining analyzed the pull-down products. C The target protein BMPR2 was identified by mass spectrometry. D The western blot detected the expression of BMPR2 and its downstream proteins Smad1/5/9, p-Smad1/5/9, and Smad4. E, F Quantitative analysis of BMPR2, Smad1/5/9, p-Smad1/5/9, and Smad4 was performed by densitometry. The results represent data from three replicates, and the mean ± SD (**P < 0.01)