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. 2022 Sep 5;10:129. doi: 10.1186/s40478-022-01435-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Reduced calbindin, parvalbumin, and non-phosphorylated neurofilament expression in the Purkinje cells after traumatic brain injury. a Schematic of anterior coronal cerebellum sections (bregma-6.12). b, c Representative confocal images (b) and quantification (c) of Purkinje cells (PCs) in the red boxed area in a co-labelled for calbindin (cyan) and parvalbumin (red) in sham-injured and cFPI mice at 2, 7, and 30 dpi, scale 50 μm. d High magnification confocal images and fluorescence intensity profiles of non-phosphorylated neurofilament-H (SMI-32) expression in Purkinje cells in the sham-injured and brain-injured mice at 2, 7 and 30 dpi in green boxed areas in b, scale 20 μm. e–g Representative Western blots (e) and quantification (f, g) of SMI-32 (200kDA) and SMI-32 (160kDA) band intensity in sham-injured and cFPI mice at 2, 7 and 30 dpi (mean ± SEM; n = 5 (Sham), n = 8 (cFPI); two-tailed Student’s t-test, unpaired). dpi days post-injury