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. Author manuscript; available in PMC: 2022 Sep 6.
Published in final edited form as: Nat Cell Biol. 2021 Mar 8;23(3):232–242. doi: 10.1038/s41556-021-00644-7

Extended Data Figure 1 |. MYOF is a novel lysosomal membrane protein in PDA cells.

Extended Data Figure 1 |

a. Co-localization of T192-mRFP-3xHA and endogenous LAMP2 in KP4 cells. b. qRT-PCR analysis of MYOF and DYSF mRNA levels across 10 human PDA cell lines and 3 non-PDA cell lines. Data is representative of n=3 biological replicates. c,d. Immuno-fluorescence staining of MYOF-HA (green) and LAMP2 (red) in PDA cell lines (MiaPaca, Patu8902 and Panc0203) (c) and non-PDA (U2OS and HPDE) cell lines (d). Arrowheads show examples of co-localization. e. Treatment of KP4, MiaPaCa and PaTu8988T cells with 75nM BafA1 for the indicated times causes an increase in p62 levels but not MYOF or DYSF. Graph shows the quantification of normalized fold change relative to LAMP1, averaged from 3 independent experiments. Data are mean ± s.d. Scale, 20μm for all panels.