. 2000 Mar;182(6):1706–1713. doi: 10.1128/jb.182.6.1706-1713.2000

TABLE 2.

Characteristics of mutants with insertions in RosR-regulated loci

Mutant	rosR genotype^g	GUS activity (nmol of product min⁻¹ mg of protein⁻¹) (mean ± SD)	Flanking DNA sequences^a	Sequence similarity of region flanking transposon insertion [protein, organism (probable function), significance]	Characteristics
MB001	rosR mutant	19,748 ± 690	AF116349, AF116350	PssK, R. leguminosarum (polysaccharide polymerization protein)^c ^e E = 3e-29	41×^h negative regulation
	rosR⁺	477 ± 81
MB002	rosR mutant	4,708 ± 441	AF116351, AF116352	Oac, bacteriophage Sf6 (O-antigen acetylase; lipopolysaccharide modification)^c ^e, E = 5e-15; NodX, R. leguminosarum bv. vicea (sugar acetylase)^c ^e, E = 1e-12	56× negative regulation
	rosR⁺	84 ± 6

MB004	rosR mutant	3,442 ± 466	AF116353, AF116354	AAB63465, Caenorhabditis elegans (O-linked GlcNAc transferase)^c ^e, E = le-08	12× negative regulation
	rosR⁺	290 ± 16
MB005	rosR mutant	27 ± 7	AF116355, AF116356	No significant similarity	7× negative regulation
	rosR⁺	4 ± 3
MB006	rosR mutant	70 ± 4	L13618	AAB07742; R. etli (putative coproporphyrinogen III oxidase; heme synthesis)^c ^e	4× positive regulation
	rosR⁺	247 ± 38
MB009	rosR mutant	1,738 ± 64	AF116357, AF116358	AAB90655, Archaeoglobus fulgidus (putative dolichol-P-glucose synthase)^c ^f, E = 0.002	17× negative regulation
	rosR⁺	105 ± 5
MB010	rosR mutant	1,512 ± 118	AF116359, AF116360	CscR, E. coli (repressor of sucrose degradation operon)^c ^e, E = 6e-05	8× negative regulation
	rosR⁺	193 ± 22
MB011	rosR mutant	4,336 ± 133	AF116361, AF116362	ORF6 in fasciation locus, R. fascians (may be involved in cytokinin synthesis)^b ^e, E = 9e-25	38× negative regulation
	rosR⁺	115 ± 9
MB012	rosR mutant	1,363 ± 331	AF116363, AF116364	AAB63465, C. elegans (O-linked GlcNAc transferase)^c ^e, E = le-08	10× negative regulation (217 bp upstream of MB004 insertion)
	rosR⁺	133 ± 28
MB013	rosR mutant	22,936 ± 3,936	AF116365, AF116366	No significant similarity	131× negative regulation, mucoid colony morphology
	rosR⁺	175 ± 18			131× negative regulation, mucoid colony morphology
MB014	rosR mutant	3036 ± 687	AF116367, AF116368	LipR, Streptomyces coelicolor (transcriptional activator)^b ^e, E = 2e-05	13× negative regulation
	rosR⁺	230 ± 13
MB015	rosR mutant	4,746 ± 2,065	AF116369, AF116370	ExsH, S. meliloti (endoglycanase; EPS synthesis)^c ^e, E = 5e-19	29× negative regulation
	rosR⁺	162 ± 7
MB016	rosR mutant	929 ± 87	AF116371, AF116372	AF116463, Streptomyces linocolnesis (hypothetical protein)^c ^e, E = 3e-05	16× negative regulation
	rosR⁺	60 ± 4
MB017	rosR mutant	405 ± 73	AF116373, AF116374	DegT, Bacillus stearothermophilus (membrane-bound regulatory protein)^c ^e E = 4e-57	8× negative regulation
	rosR⁺	50 ± 14
MB018	rosR mutant	467 ± 35	AF116375, AF116376	NocM, A. tumefaciens (nopaline permease)^d ^e E = 4e-05	10× negative regulation
	rosR⁺	48 ± 20
MB019	rosR mutant	13,005 ± 3,157	AF116377, AF116378	OtsA, Rhizobium sp. strain NGR234 (trehalose-phosphate synthase)^d ^e E, = 5e-06;	23× negative regulation
	rosR⁺	569 ± 62
MB020	rosR mutant	6,288 ± 171	AF116379, AF116380	No significant similarity	54× negative regulation
	rosR⁺	116 ± 8
MB021	rosR mutant	3,220 ± 502	AF116381, AF116382	ExoB, S. meliloti (UDP-glucose epimerase; EPS synthesis)^c ^e, E = 4e-59; AAC07360, Aquifex aeolicus (cellulose synthase)^b ^e, E = 2e−08	72× negative regulation
	rosR⁺	45 ± 10

MB022	rosR mutant	4,928 ± 376	AF116383, AF116384	No significant similarity	15× negative regulation
	rosR⁺	338 ± 17
MB023	rosR mutant	2,690 ± 710	AF116385, AF116386	No significant similarity	34× negative regulation
	rosR⁺	80 ± 9
MB024	rosR mutant	1,130 ± 275	AF116387, AF116388	No significant similarity	18× negative regulation
	rosR⁺	62 ± 33
MB025	rosR mutant	2,668 ± 247	AF116389, AF116390	ExoB; S. meliloti (UDP-glucose epimerase; EPS synthesis)^d ^e, E = 4e-59; AAC07360, A. aeolicus (cellulose synthase)^c ^e, E = 2e-08	38× negative regulation (602 bp downstream of MB021 insertion)
	rosR⁺	71 ± 3

MB026	rosR mutant	2,112 ± 423	AF116391, AF116392	No significant similarity	28× negative regulation
	rosR⁺	77 ± 4
MB027	rosR mutant	11,674 ± 3,490	AF116393, AF116394	ORF2 in picA locus, A. tumefaciens (affects cell surface changes due to plant cell extracts)^b ^e, E = 3e-14	22× negative regulation
	rosR⁺	542 ± 75
MB029	rosR mutant	3,294 ± 853	AF116395, AF116396	No significant similarity	10× negative regulation
	rosR⁺	327 ± 41
MB031	rosR mutant	2,796 ± 1,127	AF116397, AF116398	No significant similarity	8× negative regulation (49 bp downstream of MB029 insertion)
	rosR⁺	335 ± 24
MB032	rosR mutant	19,776 ± 6,851	AF116399, AF116400	PlyA, R. leguminosarum (polysaccharidase)^b ^e, E = 3e-11	66× negative regulation
	rosR⁺	301 ± 32
MB033	rosR mutant	1,576 ± 320	AF116401, AF116402	No significant similarity	22× negative regulation
	rosR⁺	71 ± 16
MB034	rosR mutant	1,885 ± 590	AF116403, AF116404	CbbZ, Rhodobacter sphaeroides (phosphoglycolate phosphatase)^b ^e, E = 4e-11	11× negative regulation
	rosR⁺	171 ± 14
MB035	rosR mutant	8,728 ± 2,849	AF116405, AF116406	Y4JH, Rhizobium sp. strain NGR234 (hypothetical protein on Sym plasmid)^c ^e, E = 5e-44	22× negative regulation
	rosR⁺	398 ± 100
MB036	rosR mutant	1,655 ± 397	AF116407, AF116408	LipR, Streptomyces coelicolor (transcriptional activator)^b ^e, E = 2e-05	20× negative regulation (312 bp downstream of MB014 insertion)
	rosR⁺	84 ± 17
MB037	rosR mutant	3,262 ± 938	AF116409, AF116410	ExoB, S. meliloti (UDP-glucose epimerase; EPS synthesis)^d ^e, E = 4e-59; AAC07360, A. aeolicus (cellulose synthase)^c ^e, E = 2e-08	51× negative regulation (1,203 bp downstream of MB021 insertion)
	rosR⁺	63 ± 30

MB038	rosR mutant	6,190 ± 2,527	AF116411, AF116412	CelR2, R. leguminosarum bv. trifolii (regulator of genes for cellulose synthesis)^c ^e, E = 2e-22	11× negative regulation
	rosR⁺	561 ± 67
MB039	rosR mutant	721 ± 256	AF116413, AF116414	PlyA, R. leguminosarum (polysaccharide)^b ^e, E = 3e-11	105× negative regulation (164 bp upstream of MB032)
	rosR⁺	7 ± 2			105× negative regulation (164 bp upstream of MB032)
MB040	rosR mutant	6,007 ± 3,312	AF116415, AF116416	PlyA, R. leguminosarum (polysaccharidase)^c ^e, E = 3e-11	198× negative regulation (302 bp downstream of MB032 insertion)
	rosR⁺	30 ± 6
MB041	rosR mutant	2,696 ± 185	AF116417, AF116418	PlyA, R. leguminosarum (polysaccharidase)^b ^f, E = 3e-11	36× negative regulation (164 bp upstream of MB032 insertion)
	rosR⁺	75 ± 16
MB042	rosR mutant	10,063 ± 1,341	AF116419, AF116420	ORF in picA locus, A. tumefaciens (affects cell surface changes due to plant cell extracts)^c ^e, E = 3e-14	52× negative regulation (1,012 bp upstream of MB027 insertion)
	rosR⁺	194 ±54
MB043	rosR mutant	4,597 ± 895	AF116421, AF116422	VirCl, A. tumefaciens (virulence determinant)^b ^f, E = 8e-27; VirD3, A. tumefaciens (unknown function)^b ^e E = 0.36	134× negative regulation
	rosR⁺	34 ± 13
MB045	rosR mutant	9,556 ± 1,291	AF116423, AF116424	HelE, Dictyostelium discoideum (similar to helicase-like transcription factor)^b ^e E = 4e-04; SlpB, Methanosarcina mazei (surface layer protein B)^b ^e E = 7e-04; PlyA, R. leguminosarum (polysaccharidase)^d ^e E = 0.013	86× negative regulation
	rosR⁺	111 ± 22


MB046	rosR mutant	18,870 ± 1,075	AF116425, AF116426	Serralysin, Pseudomonas sp. (metalloprotease)^c ^e, E = 1e-07; AlgE1, Azotobacter vinelandii (mannuronan C-5-epimerase)^c ^e, E = 4e-06	67× negative regulation
	rosR⁺	162 ± 26

MB047	rosR mutant	3,079 ± 1,165	AF116427, AF116428	No significant similarity	41× negative regulation
	rosR⁺	75 ± 12
MB048	rosR mutant	3,066 ± 286	AF116429, AF116430	BAA17459, Synechocystis sp. (hypothetical protein)^c ^e, E = 9e-50	12× negative regulation
	rosR⁺	263 ± 10
MB049	rosR mutant	2,456 ± 168	AF116431, AF116432	No significant similarity	9× negative regulation
	rosR⁺	277 ± 30
MB050	rosR mutant	3,514 ± 619	AF116433, AF116434	METRS, Methanobacterium thermoautotrophicum (methionyl-tRNA synthetase)^c ^f, E = 4e-29	13× negative regulation
	rosR⁺	265 ± 71
MB051	rosR mutant	1,442 ± 186	AF116435, AF116436	No significant similarity	82× negative regulation
	rosR⁺	18 ± 11
MB052	rosR mutant	1,101 ± 218	AF116437, AF116438	AF116463, Streptomyces linocolnesis (hypothetical protein)^c ^e, E = 3e-05	7× negative regulation (647 bp downstream of MB016 insertion)
	rosR⁺	155 ± 11
MB054	rosR mutant	3,427 ± 904	AF116439, AF116440	CAA94113; C. elegans (hypothetical protein)^c ^f, E = 0.015; CAA20616, S. coelicolor (putative lyase) ^d ^f, E = 0.077	7× negative regulation
	rosR⁺	521 ± 83
MB055	rosR mutant	10,025 ± 713	AF116441 AF116442	PrsD, R. leguminosarum (ATP-binding cassette transporter)^c ^e, E = 3e-86	8× negative regulation
	rosR⁺	1,250 ± 79
MB056	rosR mutant	8,995 ± 2,012	AF116443, AF116444	No significant similarity	6× negative regulation
	rosR⁺	1,493 ± 103
MB057	rosR mutant	1,478 ± 289	AF116445, AF116446	No significant similarity	9× negative regulation
	rosR⁺	159 ± 14
MB059	rosR mutant	3,852 ± 1,448	AF116447, AF116448	No significant similarity	6× negative regulation
	rosR⁺	617 ± 54
MB060	rosR mutant	4,365 ± 1,207	AF116449, AF116450	Gdh, Pantoea citrea (glucose dehydrogenase)^c ^e, E = 1e-24	63× negative regulation
	rosR⁺	69 ± 11
MB065	rosR mutant	19,362 ± 1,718	AF116451, AF116452	ExoY, Rhizobium sp. (EPS synthesis)^c ^e, E = 1e-51	118× negative regulation, mucoid colony morphology
	rosR⁺	164 ± 25			118× negative regulation, mucoid colony morphology

GenBank accession numbers are indicated, with the first number indicating the sequence from upstream of the gusA gene.

The transposon insertion is upstream of the region with similarity to the database member.

The transposon insertion is within the region with similarity to the database member.

The transposon insertion is downstream of the region with similarity to the database member.

gusA is in the same orientation as the region with similarity to the database member.

gusA is in the opposite orientation as the region with similarity to the database member.

rosR mutant, pLAFR3; rosR⁺, pH8B1.

41×, 41-fold.