FIG. 1.

Localization of NixA to the cytoplasmic membrane. Whole H. pylori cells and an isogenic nixA mutant harvested from third-day growth on sheep blood agar were fractionated into periplasmic, cytoplasmic, inner membrane (IM), and outer membrane (OM) components by cold osmotic shock, lysis in a French pressure cell, ultracentrifugation, and solubilization of the inner membrane with 0.5% Sarkosyl. Whole wild-type (WT) cells, whole NixA-deficient H. pylori cells, and 5 μg of protein from each fraction were separated by SDS–9% PAGE and immunoblotted with NixA-specific affinity-purified polyclonal antibodies.