Figure 1.

Lung-specific loss of function of Miz1 (c-Myc-interacting zinc finger protein-1) inhibits house dust mite (HDM)-induced allergic asthma in mice. Miz1(POZ)^fl/fl mice were intratracheally instilled with Ad/Null or Ad/Cre. Thirty days later, allergic asthma was induced in mice with HDM. (A–E) Production of Th2 cytokines in the BAL fluid (A), or mRNA concentrations of cytokines in whole-lung homogenates as indicated (B and E), or airway hyperresponsiveness to aerosolized metacholine in increasing concentrations (C), or serum total IgE concentrations (D) from Ad/Null- or Ad/Cre-pretreated, HDM-challenged Miz1(POZ)^fl/fl mice. n = 5–7 for A, C, and D. n = 5–7 with technical replicates for B and E. Values represent the mean ± SEM. Unpaired Student’s t test was used. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.