Figure 2.

Lung-specific loss of function of Miz1 suppresses OVA (ovalbumin)-induced allergic asthma in mice. Miz1(POZ)^fl/fl mice were intratracheally instilled with Ad/Null or Ad/Cre. Thirty days later, allergic asthma was induced in mice with OVA/alum (40). (A–D) Production of T-helper cell type 2 (Th2) cytokines in the BAL fluid (A), or mRNA concentrations of cytokines in whole-lung homogenates as indicated (B and D), or airway hyperresponsiveness to aerosolized metacholine in increasing concentrations (C) from Ad/Null- or Ad/Cre-pretreated, OVA-challenged Miz1(POZ)^fl/fl mice. n = 5–7 for (A and C). n = 5–7 with technical replicates for (B and D). Values represent the mean ± SEM. Unpaired Student's t test was used. *P < 0.05, **P < 0.01, and ***P < 0.001.