Figure 5.

Loss of function of Miz1 results in Th1 skewing. Ad/null or Ad/Cre-preinfected Miz1(POZ)^fl/fl mice, or control Miz1(POZ)^fl/fl, heterozygous SPC-Cre⁺/Miz1(POZ)^+/f, CD11c-Cre/Miz1(POZ)^fl/fl, or LysM-Cre/Miz1(POZ)^fl/fl mice were subjected to HDM- or OVA-induced allergic asthma as indicated. (A, C–G, I, and J) mRNA concentrations of Ifng, Tnf, Rantes, or Il12 in whole-lung homogenates of HDM- or OVA-treated mice as indicated. (B and H) Production of IFNγ and TNF in the BAL fluid from HDM- or OVA-treated mouse as indicated. n = 5–7 except in PBS groups in C (n = 3) and in G (3–4). Values represent the mean ± SEM. Unpaired Student's t test was used. *P < 0.05, **P < 0.01, and ***P < 0.001.