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. 2022 Jun 9;67(3):375–388. doi: 10.1165/rcmb.2021-0551OC

Figure 1.

Figure 1.

Exogenous miR-146a-5p disrupts barrier integrity and evokes lung inflammation. (A) miR-146a-5p expression in the BAL was measured at 24 hours after sham (n = 4) or CLP (n = 6) surgeries. (B–E) BAL and lung tissue from miR-146a knockout mice were collected and analyzed 18 hours after intratracheal injection of lipofectamine (Lipo) or miR-146a-5p (miR-146a). n = 10–11 per group. (B) BAL albumin concentration. (C) Enzyme-linked immunosorbent assay (ELISA) and quantitative RT-PCR analysis of cytokine in the BAL and lung tissue. (D) Neutrophils (Ly6G+CD45+) infiltration was assessed by flow cytometry. (E) Representative flow cytometry plots gated for Ly6G+ and CD45+. Data were presented as mean ± SEM and analyzed with an unpaired t test. CLP = cecal ligation and puncture; TNF = tumor necrosis factor.