Figure 6.

Cytokine array and pathway enrichment analysis identify TNFα as a possible effector responsible for TLR7-dependent endothelial injury. (A–B) Immune blot (A) and quantification of integrated intensity (B) of cytokine array in CM collected 24 hours from mutant- (50 nM) or miR-146a-5p– (50 nM) treated macrophages (WT and TLR7^−/−). (C–D) Cytokine array analysis from pooled sera (five mice in each strain) collected 24 hours after CLP procedure from WT and TLR7^−/− mice. (C) Immune blot and (D) quantification of the integrated intensity of top 30 differential cytokines. (E) TNFα is related to both TLR7 and endothelial injury. (F) Top-10 ranked pathways analyzed by KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis.