FIG. 5.

Various amounts of purified Fur were incubated with a DNA fragment containing the viuB-vibF intergenic region (10 nM concentration) and subjected to DNase I digestion. Lanes: 1, DNA fragment alone; 2, DNA fragment incubated with 10 nM Fur; 3, DNA fragment incubated with 50 nM Fur. The DNase I footprint is indicated, as is the location of the Fur binding consensus sequence shown in Fig. 4.