TABLE 1.
List of strains and plasmids used in this study
| Strain or plasmid | Description or genotype | Source or reference |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| O395 | O1 classical strain; Smr | 23 |
| DH1 | O395 tolQ::pDH235 | This study |
| DH2 | O395 tolR::pDH107 | This study |
| DH3 | O395 tolA::pDH149 | This study |
| DH4 | O395 tolB::pDH270 | This study |
| TCP2 | O395 ΔtcpA | 10 |
| DH5 | TCP2 tolQ::pDH235 | This study |
| DH6 | TCP2 tolR::pDH107 | This study |
| DH7 | TCP2 tolA::pDH149 | This study |
| 468-83 | TCP TLC attRS CTXφ− | 28 |
| RV508 | Rifr Specr; derivative of classical strain 569B | 37 |
| E. coli | ||
| Sm10λpir | thi thr leu tonA lacY supE recA::RP-2Tc::Mu Knr::λpir | 23 |
| TPS66 | Missense mutation in tolQ/F+ | 32 |
| Plasmids | ||
| pGP704 | Suicide vector; oriR6K mobRP4 Apr | 23 |
| pDH235 | Internal fragment of V. cholerae tolQ (bp 72–381) inserted into pGP704 | This study |
| pDH107 | Internal fragment of V. cholerae tolR (bp 22–292) inserted into pGP704 | This study |
| pDH149 | Internal fragment of V. cholerae tolA (bp 79–530) inserted into pGP704 | This study |
| pDH270 | Internal fragment of V. cholerae tolB (bp 36–270) inserted into pGP704 | This study |
| pBAD33 | Ara-inducible promoter vector; Cmr | 9 |
| pDH8 | V. cholerae tolQ cloned into pBAD33 | This study |
| pDH9 | V. cholerae tolQR cloned into pBAD33 | This study |
| pDH10 | V. cholerae tolR cloned into pBAD33 | This study |
| pDH11 | V. cholerae tolA cloned into pBAD33 | This study |
| pCTX-Kn | Replicative form of CTX-Knφ | 37 |
| pMW1 | pCTX-Kn ΔorfU | 37 |
| pMW2 | pCTX-Kn Δzot | 37 |