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. 2022 Sep 5;17:60. doi: 10.1186/s13024-022-00564-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Iba1 antibody immunostaining in young and aged mice with PBS or ɑ-syn pff injection. A Representative images for Iba1 immunostaining in young mouse cohort at 1-month post injection. Images are from three different mice (1, 2 and 3) of three brain regions (M1/M2 or somatosensory cortex (Neocortex), basolateral and basomedial amygdala (Amygdala), and dorsal striatum (Striatum)). Left panels are PBS (vehicle) injected and right are ɑ-syn pff injected. B Image analysis of Iba1 positive cell counts per 0.1mm² of young mouse cohort at 1-month post injection. C Iba1 immunostaining of young mouse cohort at 3-months post injection. Same format as (A). D Image analysis of Iba1 positive cell counts per 0.1mm² of young mouse cohort at 3-months post injection. E Iba1 immunostaining of aged mouse cohort at 1-month post injection. Same format as (A). F Image analysis of Iba1 positive cell counts per 0.1mm² of aged mouse cohort at 1-month post injection. G Iba1 immunostaining of aged mouse cohort at 3-months post injection. Same format as (A). H Image analysis of Iba1 positive cell counts per 0.1mm² of aged mouse cohort at 3-months post injection. I-K Comparison of image analysis of Iba1 positive cell counts per 0.1mm² in cortex (I) amygdala (J) and striatum (K) of ɑ-syn pff injected mice at 1- and 3-months post-injection in young (blue) and aged (red) mouse cohorts. Scale bars, 20 μm. Data are mean ± SEM. Unpaired t test was used. **p < 0.01; ***p < 0.001; ****p < 0.0001