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. 2022 Sep 6;8:86. doi: 10.1038/s41421-022-00450-x

Fig. 3. Formation of P4 G4 inhibits SARS-CoV-2 RNA translation and replication processes.

Fig. 3

a TMPyP4 or PDP treatment inhibits the translation of mRNA with P4 G4 by performing IVT assays. b TMPyP4 or PDP treatment inhibits the protein expression by targeting P4 G4 in HEK293T and Hela cells. c The protein expression of nsp7, nsp8 and nsp12, detected by western blotting assays. d The formation of P4 G4 inhibits the replication of RNA with P4 G4 in HEK293T cells, detected by qRT-PCR assays. e, f TMPyP4 (e) or PDP (f) treatment inhibits the replication of RNA with P4 G4 in HEK293T cells in a dose-dependent manner, detected by qRT-PCR assays. g HEK293T cells infected with P4-EGFP lentivirus were treated with or without 10 μM TMPyP4 or 2.5 μM PDP. Left, representative confocal images. Right, the relative fluorescent value. Scale bars, 100 μm. Data are shown as means ± SEM of three independent experiments, two-tailed Student’s t-test. n.s. not significant. *P < 0.05, ***P < 0.001, ****P < 0.0001.