TABLE 1.

Primer lists for gene expression studies and antisense probe preparation for In situ hybridization experiments. bp, base pairs

Genes	Forward primer (5′‐3′)	Reverse Primer (5′‐3′)	Amplicon (bp)	Annealing Temp (°C)	NCBI accession ID
For Gene expression
Tas2R1	GACCATCACAGGCATCATACA	TTGGTCAAAGTCTGCAGTAGTAA	103	57.1	XM_022680204.1
Tas2R3	CTTTGACCATGGTGCTGATTATG	GCTGCTGGTCATCTTCCTAATA	104	62.8	XM_022678463.1
Tas2R4	GAACCAACGTAACCACCAATAC	GGGCAGAGGAAGGACATATAAA	118		XM_007248674.2
Tas2R114	CAAGCTGTGCAACTGTTCTATAC	ATGGTCACCCTCATCTGTTTC	106		XM_022679999.1
GAPDH	GTGATGAACGCTCCTCTATCTTT	GTTGCTGTAGCCAAACTCATTG	104	60.2	XM_007249798.3
For in‐situ hybridization
Tas2R1	GTGCAACACAGCTCTTCATTT	TAATACGACTCACTATAGGGGTGTCTCCGCAGGTATGAAATA	103	60	XM_022680204.1
Tas2R3	CTCTCCTGGTGCTTGTGATTT	TAATACGACTCACTATAGGGTCAGCACCATGGTCAAAGTATC	104	62.1	XM_022678463.1
Tas2R4	GGGTGATGTGGAACTCCTTT	TAATACGACTCACTATAGGGGGCCGTGACTTTAGGAATGTA	118		XM_007248674.2
Tas2R114	GTGGCTGAACGTGTTCTACTAT	TAATACGACTCACTATAGGGATGGTCACCCTCATCTGTTTC	106	68	XM_022679999.1