Figure 7.

(A) Changes in AuNR scattered red light intensity during one complete revolution involving three consecutive power strokes (PS) and three consecutive catalytic dwells (D) separated by exactly 120° when, prior to data collection, the polarizer is rotated such that scattered light intensity is at a minimum during catalytic dwell-1 (D1). (B) Rotational off-set of polarizer positions when two detectors are used to observe the direction of rotation. (C) Counterclockwise rotation observed by EcF₁-ATPase-dependent rotation calculated from two detectors with off-set polarizer positions with data acquired at 10 kHz in the presence of 1 mM MgCl₂ and 2 mM ATP. (Inset) Detail of the rotational stepping of the boxed region. Horizontal lines show the 120° dwell positions. This figure was reconfigured from Spetzler et al. (2009).