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Nuclease S1 protection assays of ORFs described in this study. Lanes 1, synthetic radiolabelled oligonucleotide in the absence of mRNA or nuclease S1. Lanes 2 to 4, nuclease S1-resistant oligonucleotides after hybridization with mRNA from a virG+ strain cultured without acetosyringone (lane 2), from a virG+ strain cultured with 100 μM acetosringone (lane 3), or from a virG mutant strain cultured with 100 μM acetosyringone (lane 4).
