TABLE 2.
Synthetic oligonucleotides used in this study
Oligonucleotide | Sequence |
---|---|
For nuclease S1 protection assays | |
virB1 | 5′-CCCCGATCTCTTAAACATACCTTATCTCCTTAGCTCGCCCTGG-3′ |
rpoD | 5′-CCCTTCGCGCGACAGAAGCTCGACGGAACCCATTTCTATAC-3′ |
virD5 | 5′-GCCCTCAATACCAAATCTTTCCAAGTCGGTGGCTCCGCCTCGGCCCCTGA-3′ |
virE3 | 5′-GGGGCTCTCCAGTCTGGTTTGCCGCTTGAGCGCTCTCCT-3′ |
ORF12 | 5′-GGATTGCAAGCTCCACCCCCTGATCCTCGAGGTGAAGTAGGAAGGC-3′ |
ORF13 | 5′-CGGCGTCGACGGCATTCTCCGCATCGCGACGGTGGTAAATA-3′ |
ORF14 | 5′-CGGGACCTCCGTCGTCCGGCGTAATGCAACCAATTTGG-3′ |
ORF15 | 5′-GGGCTGCATCGCCGGATTGGTTCGCCGAAGGACCGGAACCCTTTT-3′ |
ORF16 | 5′-GCCGAGACTTCTTCCCATTGCCGTTTCAGGTCAAA-3′ |
ORF17 | 5′-GGCCGAGCGGCTCGCTTGGGGTCAGGTTCAGTTAGG-3′ |
virP | 5′-GGATTGCAAGCTCCACCCCCTGATCCTCGAGGTGAAGTAGGAAGGC-3′ |
virR | 5′-GCGCCACGGCCCACGGTTCCGAAATTTCAAGTGTGCATAGTGGCCCACCG-3′ |
For PCR amplification | |
virD5 | 5′-AGAATTCCCGGCCCACGTGGAAAGACC-3′ |
5′-ATCTAGACGGCTCGCCTAAGGGAACACC-3′ | |
virE3 | 5′-CCAGACTGGAGAGCCCCG-3′ |
5′-CCATTTTCGTCGCGCTCCC-3′ | |
ORF12-lacZ | 5′-CCGAATTCGTTCCTCGTGACTTGCCCCC-3′ |
5′-CGTCTAGAGACGGCGTCGACGGCATTCTC-3′ | |
ORF16-lacZ | 5′-GGGGTACCGCGCCGCCGTCTTCGTGTCCG-3′ |
5′-GCTCTAGAAACCACGCCGCCGGAATCAGG-3′ | |
virP-lacZ | 5′-CGAATTCGATGGTACGTTGGCAGACAGTGGT-3′ |
5′-CCTCTAGACTTATTAATCCTTTTGCCCTTCC-3′ | |
virR-lacZ | 5′-GGAATTCTGTCATGCACTCAACCAGATT-3′ |
5′-GGTCTAGATCAGCGGGACTTAGTAGGACGATA-3′ |