TABLE 3.

Comparison of mucin-desulfating sulfatase (MDS) activities in the periplasm of mucin-grown Prevotella strain RS2 and the periplasm of chondroitin sulfate-grown B. thetaiotaomicron containing plasmid pVGA-3 with an mdsA insert

Conditions	Periplasmic MDS activity, nmol/min/mg of protein (% inhibition)
	Prevotella strain RS2	B. thetaiotaomicron
Glucose-6-sulfate substrate	24	82
Glucose-6-sulfate substrate + 2 mM N-acetylglucosamine-6-phosphate	3 (87)	12 (85)
Glucose-6-sulfate substrate + 3 mM N-acetylglucosamine	10 (58)	51 (38)