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. 2022 Jul 29;323(3):H577–H584. doi: 10.1152/ajpheart.00322.2022

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Copyright © 2022 The Authors

Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.

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Figure 2. — Characterization of exosome-related protein markers and molecular masses in vesicles isolated from adipose-tissue culture media. Protein content of Alix (100 and 75 kDa; A) and TSG101 (∼50 kDa; B) in samples of Exo-utPVAT and Exo-OVAT derived from nonpregnant and pregnant rats. Representative chromatograms fast protein liquid chromatography (FPLC) for Exo-utPVAT (C) and Exo-OVAT samples (D), demonstrating time at which the protein eludes in the FPLC column. Exo-OVAT, exosome-like extracellular vesicles derived from periovarian adipose tissue; Exo-utPVAT, exosome-like extracellular vesicles derived from uterine perivascular adipose tissue; utPVAT, uterine perivascular adipose tissue.