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. 2022 Jul 29;323(3):H577–H584. doi: 10.1152/ajpheart.00322.2022

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Copyright © 2022 The Authors

Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.

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Figure 3. — Trafficking of uterine perivascular adipose tissue-derived exosome-like vesicles into adjacent uterine vascular smooth muscle cells in a pregnant rat. Immunocytochemical images of untreated utVSMCs (A), utVSMCs treated with unlabeled exosomes (B), utVSMCs treated with membrane staining solution DiO (C), utVSMCs treated with exosome-free supernatant (D), and utVSMCs treated with labeled Exo-utPVAT (E). Inset: Exo-utPVAT are labeled green (63× Z-stack max intensity). Scale bar = 200 µm. Tissues were derived from a pregnant rat (gestational age = 16 days, term = 22–23 days). Exo-utPVAT, exosome-like extracellular vesicles derived from uterine perivascular adipose tissue; utPVAT, uterine perivascular adipose tissue; utVSMCs, uterine vascular smooth muscle cells.