Table 3.
Mean slopes for the force-flow relationships in Fig. 3
| Figure | Fuel | Control Mitochondria |
Ischemia Mitochondria |
|||
|---|---|---|---|---|---|---|
| Slope | r 2 | Slope | r 2 | |||
| Eh NAD/NADH, mV | 3A | G + M | 21.0 ± 1.3 | 0.93 ± 0.03 | 10.3 ± 1.5* | 0.97 ± 0.01 |
| 3B | P + M | 18.0 ± 3.7 | 0.91 ± 0.08 | 10.4 ± 1.7* | 0.94 ± 0.06 | |
| ΔΨ, mV | 3C | G + M | 39.9 ± 4.4 | 0.97 ± 0.01 | 22.8 ± 2.7* | 0.96 ± 0.01 |
| 3D | P + M | 43.2 ± 5.6 | 0.98 ± 0.01 | 26.0 ± 4.0* | 0.96 ± 0.01 | |
| ΔGATPe, kcal/mol | 3E | G + M | 180.0 ± 19.4 | 0.97 ± 0.01 | 84.4 ± 9.8* | 0.98 ± 0.01 |
| 3F | P + M | 192.1 ± 21.4 | 0.98 ± 0.00 | 87.8 ± 12.9* | 0.97 ± 0.01 | |
Values are means ± SE; n = 4–12 rats per condition. The slopes of the Eh NAD/NADH: JO, ΔΨ:JO, and ΔGATPe: JO relationships (Fig. 3, A and B, C and D, and E and F, respectively) were lower in mitochondria following ischemia, demonstrating a blunted sensitivity to mitochondrial driving forces following ischemia. G, glutamate; M, malate; P, pyruvate. *P < 0.05, significantly different from control, two-tailed t test.