Table 4.
Mean slopes for the force-flow relationships in Fig. 4
| Figure | Fuel | Control Mitochondria |
Ischemia Mitochondria |
|||
|---|---|---|---|---|---|---|
| Slope | r 2 | Slope | r 2 | |||
| L OxPhos | 4A | G + M | 74.2 ± 7.8 | 0.98 ± 0.01 | 30.4 ± 2.2* | 0.98 ± 0.01 |
| 4B | P + M | 93.5 ± 11.3 | 0.98 ± 0.01 | 42.6 ± 7.0* | 0.97 ± 0.01 | |
| L fuel/DH | 3A | G + M | 401.4 ± 58.4 | 0.93 ± 0.03 | 195.3 ± 34.2* | 0.97 ± 0.01 |
| 3B | P + M | 394.0 ± 60.9 | 0.91 ± 0.05 | 303.6 ± 79.0* | 0.97 ± 0.01 | |
| L ETC | 4C | G + M | 275.9 ± 50.2 | 0.94 ± 0.04 | 107.4 ± 19.2* | 0.97 ± 0.02 |
| 4D | P + M | 252.2 ± 73.0 | 0.94 ± 0.02 | 142.9 ± 39.8* | 0.93 ± 0.04 | |
| L ATP synth + ANT | 4E | G + M | 109.3 ± 19.4 | 0.98 ± 0.01 | 40.7 ± 3.7* | 0.98 ± 0.01 |
| 4F | P + M | 166.9 ± 29.0 | 0.94 ± 0.02 | 55.7 ± 14.6* | 0.96 ± 0.02 | |
| L H2O2 | 6E | G + M | 70.4 ± 22.2 | 0.88 ± 0.04 | 72.3 ± 20.0 | 0.90 ± 0.05 |
| 6F | P + M | 28.4 ± 7.2 | 0.90 ± 0.80 | 45.2 ± 15.4 | 0.89 ± 0.08 | |
Values are means ± SE; n = 4–6 rats per condition. Control of mitochondrial respiration was altered after ischemia, with ischemia mitochondria demonstrating a ubiquitous reduced activity across the oxidative phosphorylation pathway. Ischemia decreased the slope, or the pathway activity, of 1) the fuel dehydrogenases, 2) the electron transport chain, and 3) ATP synthase and the adenine nucleotide translocase. However, the slope of the ΔGredox − ΔGΨ:H2O2 relationship was unchanged after ischemia, indicating that a given change in driving force would result in the same increase in H2O2 release in both control and ischemia mitochondria. G, glutamate; M, malate; P, pyruvate. *P < 0.05, significantly different from control, two-tailed t test.