Figure 6.

Differential exon usages in aging hearts. A–C: exon usages in sex-adjusted comparisons between 20-mo (blue) and 4-mo (red) mouse hearts. Y-axis shows normalized read counts across disjoint exonic parts in DEXSeq after adjusting for gene-level differential expression. The disjoint exonic parts in DEXSeq are mapped to annotated exons in the Ensembl gene model below with the genomic coordinates labeled. Exonic parts with significant differential usage in 20-mo vs. 4-mo mouse hearts are colored in purple. D: intersection between genes with differential exon usage (DEU) at 20 mo vs. 4 mo (DEU_AGE) at 10% FDR with genes with differential gene expression (DGE) across age and sex comparisons. E: dot plot showing the enrichment ratio and adjusted P values of overrepresented Reactome pathways among genes with age-associated exon usage difference. Exon usage changes are enriched along RNA metabolism, ribosomal, and mitochondrial metabolic pathways. F: gene concept network showing top 15-enriched Reactome terms and their associated genes with significant differences in exon usage in 20-mo vs. 4-mo hearts.