Figure 2.

Satellite cell depletion does not affect cross-sectional area in diaphragm with or without hypoxia. Representative image of dystrophin antibody staining on mouse diaphragm; scale bar represents 150 µm (A). Pooled cross-sectional area of vehicle-treated and tamoxifen-treated adult (B) and aged (C) diaphragm. ^Main effect for hypoxia (P = 0.002); #main effect for age (P < 0.0071). Adult normoxia veh group n = 9, adult normoxia tam group n = 5, adult hypoxia veh group n = 7, adult hypoxia tam group n = 8, aged normoxia veh group n = 5, aged normoxia tam group n = 4, aged hypoxia veh group n = 5, aged hypoxia tam group n = 5. Differences in cross-sectional area were tested using two-way ANOVA with Šídák’s multiple comparison correction. Age effects were detected using three-way ANOVA. Error bars represent SE. tam, tamoxifen; veh, vehicle.