Figure 6.

Efficacy of DB combined with the conventional chemotherapeutic drug, ADM, in a MDA-MB-231 cell line-derived tumor mouse model. (A) Mouse experimental scheme map. (B) Changes in tumor volume in different groups of mice during treatment. Calculation formula: tumor volume=shortest diameter² × longest diameter/2. ^*P<0.05, ^**P<0.01 and ^****P<0.0001 vs. the control. (C) Changes in body weight in different groups of mice during treatment. (D) Images of tumors removed from nude mice after 21 days of treatment. (E) Tumor tissue weight of mice in different groups (control, DB, ADM and DB + ADM) after 21 days of treatment. (F) On day 21, all mice were sacrificed, and tumors were isolated for histopathological examination with TUNEL, Ki-67 and LC3 B staining assays (scale bar, 100 µm). The dose of DB and ADM was 10 mg/kg. DB, dicitrinone B; ADM, Adriamycin; LC3 B, microtubule associated protein 1 light chain 3 beta. ^***P<0.001 vs. the control. (G) Relative expression quantification of histopathological examination with TUNEL, Ki-67 and LC3 B staining assays. ^*P<0.05 and ^***P<0.001.