Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 16;50(4):124. doi: 10.3892/ijmm.2022.5180

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

COMM-SUS cells resist oxidative stress by NADPH accumulation via PPP. (A) Schematic diagram of the main enzymes of the pentose phosphate pathway, fatty acid synthesis pathway and folate metabolism pathway. The expression levels of the enzymes were interfered with by corresponding siRNA. (B) The expression of the main enzymes was measured in COMM-1 AD and COMM-1 SUS cells using reverse transcription-quantitative PCR. (C) The amount of intracellular NADH and NADPH was evaluated after suppressing enzyme expression. Significance was determined using a Student's t-test or one-way ANONA (^*P<0.05, ^**P<0.01, ^***P<0.001 and ^****P<0.0001; ns, not significant, P>0.05). COMM, Chinese oral mucosal melanoma; COMM-AD, cells with adhesive morphology; COMM-SUS, cells grown in suspension; PGD, 6-phosphogluconate dehydrogenase; G6PD, glucose-6-phosphate dehydrogenase.