Figure 6. Bidirectional optogenetic manipulation of interneuron (IN) activity affects spike time tiling coefficient (STTC) in the developing mouse medial prefrontal cortex (mPFC).

(A and B) 2D kernel density plots displaying STTC before IN optogenetic manipulation (STTCpre) and STTC during optogenetic manipulation (STTCstim) during IN activation (A) and inhibition (B) (n=10,173 spike train pairs and 19 mice, n=9778 spike train pairs and 40 mice, respectively). (C and D) Average STTCpre and STTCstim during IN activation (C) and inhibition (D) over distance (n=10,173 spike train pairs and 19 mice, n=9778 spike train pairs and 40 mice, respectively). In (C and D) data are presented as mean ± SEM. Asterisks in (A and B) indicate significant effect of IN activation and inhibition, respectively. Asterisks in (C and D) indicate significant effect of IN activation*distance and IN inhibition*distance interaction, respectively. ***p<0.001. Linear mixed-effect models. For detailed statistical results, see Supplementary file 1.

Figure 6—figure supplement 1. Bidirectional optogenetic manipulation of interneuron (IN) activity effects on spike time tiling coefficient (STTC) depends on spatial configurations and age.

(A and B) Scatter plot displaying Pearson (A) and Spearman (B) correlation coefficients for STTC before IN optogenetic manipulation (STTCpre) and STTC during optogenetic manipulation (STTCstim) with STTC computed on baseline data over lags (n=19,951 spike train pairs and 59 mice). (C) Multivariate linear regression coefficients as a function of STTC lag (n=19,951 spike train pairs and 59 mice). In (C) regression coefficients are presented as mean and 95% CI. Linear mixed-effect models. For detailed statistical results, see Supplementary file 1.